EasyStep Human PCT (Procalcitonin) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK003ES-48, ELK003ES-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: Pro-Calcitonin
Species: Human
Assay Type: Sandwich
Sensitivity: 1.5 pg/mL
Standard: 500 pg/mL
Detection Range: 7.81-500 pg/mL
Sample Type: Serum, Plasma
Assay length: 1.5h
Research Areas: Infection immunity; Hormone metabolism
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PCT, and the Human PCT standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human PCT. After TMB substrate solution is added, only those wells that contain Human PCT and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PCT in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only.