Product Description

EasyStep Human PG (Progesterone) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK045ES-48, ELK045ES-96, ELK045ES-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Human

Alternate Names: P4, Pregn-4-Ene-3,20-Dione

Sensitivity: 0.7 ng/mL

Assay Type: Competitive Inhibition

Standard: 100 ng/mL

Detection Range: 1.56-100 ng/mL

Sample Type: Serum, Plasma

Assay Length: 1.5h

Research Area: Endocrinology, Reproductive Science, Hormone Metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PG antigen, and the Human PG standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human PG to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human PG in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only