EasyStep Human T4 (Thyroxine) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK054ES-48, ELK054ES-96, ELK054ES-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: 3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine
Assay Type: Competitive Inhibition
Sensitivity: 7 ng/mL
Standard: 600 ng/mL
Detection Range: 9.38-600 ng/mL
Sample Type: Serum, plasma
Assay Length: 1.5h
Research Areas: Endocrinology; Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human T4 antigen, and the Human T4 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human T4 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human T4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only