Product Description

EasyStep Human TF (Tissue Factor) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK073ES-48, ELK073ES-96, ELK073ES-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TF, and the Human TF standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human TF. After TMB substrate solution is added, only those wells that contain Human TF and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TF in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: CD142, FIII, F3, TFA, Thromboplastin, Coagulation Factor III

Reactivity: Human

Sample Type: Serum, Plasma

Assay Type: Sandwich

Research Areas: Hematology, Cardiovascular Biology

Assay Length: 1.5h

Sensitivity: 0.12 pg/mL

Standard: 800 pg/mL

Detection Range: 12.5-800 pg/mL

Research Use Only