Product Description

EasyStep Human TIMP1 (Tissue Inhibitors Of Metalloproteinase 1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK086ES-48, ELK086ES-96, ELK086ES-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Human

Alternate Names: EPO, CLGI, EPA, HCI, Erythroid Potentiating Activity, Collagenase Inhibitor, Metalloproteinase inhibitor 1, Fibroblast collagenase inhibitor

Sensitivity: 43.53 pg/mL

Assay Type: Sandwich

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: Serum, Plasma

Assay Length: 1.5h

Research Area: Tumor Immunity, Infection Immunity, Cardiovascular Biology, Hepatology, Hormone Metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TIMP1, and the Human TIMP1 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human TIMP1. After TMB substrate solution is added, only those wells that contain Human TIMP1 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TIMP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only