Product Description

EasyStep Human TRF (Transferrin) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK066ES-48, ELK066ES-96, ELK066ES-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TRF, and the Human TRF standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human TRF. After TMB substrate solution is added, only those wells that contain Human TRF and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TRF in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: TF, Siderophilin, Serotransferrin, Beta-1 metal-binding globulin

Reactivity: Human

Sample Type: Serum, Plasma

Assay Type: Sandwich

Research Areas: Tumor Immunity, Infection Immunity, Immune Molecule

Assay Length: 1.5h

Sensitivity: 11.9 ng/mL

Standard: 1000 ng/mL

Detection Range: 15.63-1000 ng/mL

Research Use Only