EasyStep Human VCAM1 (Vascular Cell Adhesion Molecule 1) ELISA Kit - ELK043ES

EasyStep Human VCAM1 (Vascular Cell Adhesion Molecule 1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK043ES-48, ELK043ES-96, ELK043ES-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: CD106; INCAM-100; L1CAM

Assay Type: Sandwich

Sensitivity: 0.87 ng/mL

Standard: 80 ng/mL

Detection Range: 1.25-80 ng/mL

Sample Type: Serum, plasma

Assay Length: 1.5h

Research Areas: CD and Adhesion molecule; Tumor immunity; Infection immunity;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VCAM1, and the Human VCAM1 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VCAM1. After TMB substrate solution is added, only those wells that contain Human VCAM1 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VCAM1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P19320

Research Use Only