EasyStep Human VEGF165 (Vascular Endothelial Growth Factor 165) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK027ES-48, ELK027ES-96, ELK027ES-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: VEGF165
Assay Type: Sandwich
Sensitivity: 15 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma
Assay Length: 1.5h
Research Areas: Cytokine; Tumor immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VEGF165, and the Human VEGF165 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VEGF165. After TMB substrate solution is added, only those wells that contain Human VEGF165 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VEGF165 in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only