Product Description

EasyStep Human VEGFA (Vascular Endothelial Growth Factor A) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK033ES-48, ELK033ES-96, ELK033ES-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VEGFA, and the Human VEGFA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VEGFA. After TMB substrate solution is added, only those wells that contain Human VEGFA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VEGFA in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: VEGF, VEGF-A, VPF, Vascular Endothelial Cell Growth Factor A, Vascular Permeability Factor

Reactivity: Human

Sample Type: Serum, Plasma

Assay Type: Sandwich

Research Areas: Cytokine, Apoptosis, Tumor Immunity

Assay Length: 1.5h

Sensitivity: 31 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.2-5000 pg/mL

UniProt: P15692

Research Use Only