Product Description

EasyStep Rat E2 (Estradiol) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK022ES-48, ELK022ES-96, ELK022ES-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat E2 antigen, and the Rat E2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat E2 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: 17B-Estradiol, Oestradiol, Beta-Estradiol

Reactivity: Rat

Sample Type: Serum

Assay Type: Competitive Inhibition

Research Areas: Endocrinology, Reproductive Science, Hormone Metabolism

Assay Length: 1.5h

Sensitivity: 25 pg/mL

Standard: 1600 pg/mL

Detection Range: 25-1600 pg/mL

Research Use Only