EasyStep Rat NPT (Neopterin) ELISA Kit - ELK101ES

EasyStep Rat NPT (Neopterin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK101ES-48, ELK101ES-96, ELK101ES-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Neopterin

Assay Type: Competitive Inhibition

Sensitivity: 0.4 nmol/L

Standard: 30 nmol/L

Detection Range: 0.469-30 nmol/L

Sample Type: Serum, plasma

Assay Length: 1.5h

Research Areas: Cytokine; Infection immunity;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat NPT antigen, and the Rat NPT standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat NPT to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat NPT in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only