EasyStep Rat PG (Progesterone) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK047ES-48, ELK047ES-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: P4; Pregn-4-Ene-3,20-Dione
Species: Rat
Assay Type: Competitive Inhibition
Sensitivity: 0.7 ng/mL
Standard: 100 ng/mL
Detection Range: 1.56-100 ng/mL
Sample Type: Serum, Plasma
Assay length: 1.5h
Research Areas: Endocrinology; Reproductive science; Hormone metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat PG antigen, and the Rat PG standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat PG to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat PG in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only.