EasyStep Rat PG (Progesterone) ELISA Kit - ELK047ES

EasyStep Rat PG (Progesterone) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK047ES-48, ELK047ES-96, ELK047ES-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: P4; Pregn-4-Ene-3,20-Dione

Assay Type: Competitive Inhibition

Sensitivity: 0.7 ng/mL

Standard: 100 ng/mL

Detection Range: 1.56-100 ng/mL

Sample Type: Serum, plasma

Assay Length: 1.5h

Research Areas: Endocrinology; Reproductive science; Hormone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat PG antigen, and the Rat PG standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat PG to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat PG in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only