Product Description

EasyStep Rat VE-Cadherin (Vascular Endothelial Cadherin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK104ES-48, ELK104ES-96, ELK104ES-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Rat

Alternate Names: CD144, 7B4, Cadherin 5 Type 2, VE-Cadherin, Cadherin,Vascular Endothelial, CDH5, Cadherin 5

Sensitivity: 0.032 µg/mL

Assay Type: Sandwich

Standard: 10 µg/mL

Detection Range: 0.16-10 µg/mL

Sample Type: Serum, Plasma

Assay Length: 1.5h

Research Area: Cd And Adhesion Molecule, Tumor Immunity, Infection Immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat VE-Cadherin, and the Rat VE-Cadherin standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat VE-Cadherin. After TMB substrate solution is added, only those wells that contain Rat VE-Cadherin and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat VE-Cadherin in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only