EasyStep Rat VEGFR2 (Vascular Endothelial Growth Factor Receptor 2) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK083ES-48, ELK083ES-96, ELK083ES-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: CD309; FLK1; VEGFR; KDR; A Type III Receptor Tyrosine Kinase; Kinase Insert Domain Receptor; Kinase Insert Domain Receptor; Fetal Liver Kinase-1
Assay Type: Sandwich
Sensitivity: 157.5 pg/mL
Standard: 50 ng/mL
Detection Range: 0.78-50 ng/mL
Sample Type: Serum, plasma
Assay Length: 1.5h
Research Areas: Signal transduction; CD and Adhesion molecule; Tumor immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat VEGFR2, and the Rat VEGFR2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat VEGFR2. After TMB substrate solution is added, only those wells that contain Rat VEGFR2 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat VEGFR2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only