Product Description

EDG-4 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES7976-50, ES7976-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: lysophosphatidic acid receptor 2(LPAR2) Homo sapiens This gene encodes a member of family I of the G protein-coupled receptors, as well as the EDG family of proteins. This protein functions as a lysophosphatidic acid (LPA) receptor and contributes to Ca2+ mobilization, a critical cellular response to LPA in cells, through association with Gi and Gq proteins. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Monkey

Host: Rabbit

Applications: WB, IF, ELISA

Alternate Names: LPAR2, EDG4, LPA2, Lysophosphatidic acid receptor 2, LPA receptor 2, LPA-2, Lysophosphatidic acid receptor Edg-4

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human EDG4. AA range:271-320

Isotype: IgG

Target: EDG-4

Specificity: EDG-4 Polyclonal Antibody detects endogenous levels of EDG-4 protein.

Dilution: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed most abundantly in testes and peripheral blood leukocytes with less expression in pancreas, spleen, thymus and prostate, Little or no expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney, ovary, small intestine, or colon,

Observed Band (KD): 39kD

Fields: Rap1 signaling pathway, Phospholipase D signaling pathway, Neuroactive ligand-receptor interaction, PI3K-Akt signaling pathway, Regulation of actin cytoskeleton, Pathogenic Escherichia coli infection, Pathways in cancer

Function: Function: Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Plays a key role in phospholipase C-beta (PLC-beta) signaling pathway
Miscellaneous: PubMed: 9525886 cDNA clone has a guanine nucleotide deletion that causes a frameshift near its C-terminal coding region. This likely reflects a somatic mutation in the ovary tumor cells from which the cDNA was isolated and may have altered the function of the encoded receptor, and contributed to transformation of the original ovary cells that formed a tumor
Similarity: Belongs to the G-protein coupled receptor 1 family
Subunit: Interacts with SLC9A3R2/NHERF2, MAGI3 and PLCB3
Tissue Specificity: Expressed most abundantly in testes and peripheral blood leukocytes with less expression in pancreas, spleen, thymus and prost

Subcellular Location: Cell surface Cell membrane, Multi-pass membrane protein Prior to LPA treatment found predominantly at the cell surface but in the presence of LPA colocalizes with RALA in the endocytic vesicles

Storage and Stability: -20°C/1 year

Gene Name: LPAR2

Protein Name: Lysophosphatidic acid receptor 2

Mouse SwissProt Number: Q9JL06

Research Use Only