Product Description

EGF Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES8651-50, ES8651-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016],

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: Pro-epidermal growth factor (EGF) [Cleaved into: Epidermal growth factor (Urogastrone)]

Clonality: Polyclonal

Immunogen: Synthetic peptide from human protein at AA range: 1000-1060

Isotype: IgG

Target: EGF

Specificity: The antibody detects endogenous EGF

Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in kidney, salivary gland, cerebrum and prostate,

Observed Band (KD): 130kD

Fields: EGFR tyrosine kinase inhibitor resistance, MAPK signaling pathway, ErbB signaling pathway, Ras signaling pathway, Rap1 signaling pathway, Calcium signaling pathway, HIF-1 signaling pathway, FoxO signaling pathway, Phospholipase D signaling pathway, PI3K-Akt signaling pathway, Focal adhesion, Gap junction, JAK-STAT signaling pathway, Regulation of actin cytoskeleton, Hepatitis C, Human papillomavirus infection, Pathways in cancer, Chemical carcinogenesis - receptor activation, Chemical carcinogenesis - reactive oxygen species, Colorectal cancer, Pancreatic cancer, Endometrial cancer, Glioma, Prostate cancer, Melanoma, Bladder cancer, Non-small cell lung cancer, Breast cancer, Gastric cancer, Choline metabolism in cancer, PD-L1 expression and PD-1 checkpoint pathway in cancer

Function: Disease: Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4) [MIM: 611718], also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes
Function: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6
Online Information: Epidermal growth factor entry,Similarity: Contains 9 EGF-like domains
Similarity: Contains 9 LDL-receptor class B repeats
Tissue Specificity: Expressed in kidney, salivary gland, cerebrum and prostate

Subcellular Location: Membrane, Single-pass type I membrane protein

Storage and Stability: -20°C/1 year

Gene Name: EGF

Protein Name: Pro-epidermal growth factor (EGF) [Cleaved into: Epidermal growth factor (Urogastrone)]

Mouse Gene ID: 13645

Mouse SwissProt Number: P01132

Research Use Only