Product Description

ELISA Kit for Anti-Interferon Gamma Antibody (Anti-IFNg)

Sizes: 24T, 48T, 96T

Catalogue Numbers: AEA049Hu-24, AEA049Hu-48, AEA049Hu-96

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Featured Series Function: Detects protein (antibody)

Method: Colormetric

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Interferon Gamma Antibody (Anti-IFNg) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Interferon Gamma Antibody (Anti-IFNg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reactivity: Human

Alternate Names: IFN-G, IFG, IFI, INFr, IFN, Immune Interferon

Target: Interferon Gamma,

Assay Length: 2.5h

Sample Size: 50uL

Sample Type: Serum

Detection Method: Competitive Inhibition

Detection Range: 3.12-200ng/mL

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Interferon Gamma Antibody (Anti-IFNg) were tested 20 times on one plate, respectively.Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Interferon Gamma Antibody (Anti-IFNg) were tested on 3 different plates, 8 replicates in each plate.CV(%) = SD/meanX100

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Assay Procedure: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 5 times; 5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 6. Add 50µL Stop Solution. Read at 450nm immediately.

Specificity: Reactive with Human IFNg / Interferon Gamma

Sensitivity: 1.22ng/mL

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Shelf Life: 12 months

Gene ID: 3458

UniProt: P01579

Research Use Only