ELISA Kit for Diablo Homolog (DIABLO) - USEJ266Hu

ELISA Kit for Diablo Homolog (DIABLO)

Size: 96T

Catalogue Number: USEJ266Hu-96

Citations, Manuals and MSDS Available upon request.

Target: Diablo Homolog (DIABLO)

Alternative Names: SMAC; DIABLO-S; Direct IAP-binding protein with low pI; Second mitochondria-derived activator of caspase

Target Species: Human

Detection range: 0.156-10ng/mL

Sensitivity: 0.055ng/mL

Uniprot: Q9NR28

Gene ID: 56616

Featured Series Function: Detects protein (regular version)

Recommended/Predicted Sample Types: Tissue Homogenates, Cell Lysates and other Biological Fluids

Assay Time: 3h

Method: Colormetric

Specificity: Reactive with Human DIABLO / Diablo Homolog

Detection principle: Double-antibody Sandwich

Sample Size: 100uL

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Diablo Homolog (DIABLO).

No significant cross-reactivity or interference between Diablo Homolog (DIABLO) and analogues was observed.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Diablo Homolog (DIABLO) were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Diablo Homolog (DIABLO) were tested on 3 different plates, 8 replicates in each plate.

CV(%) = SD/meanX100

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.

To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards;

2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;

3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;

4. Aspirate and wash 3 times;

5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;

6. Aspirate and wash 5 times;

7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;

8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Diablo Homolog (DIABLO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Diablo Homolog (DIABLO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Diablo Homolog (DIABLO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Diablo Homolog (DIABLO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Signal transduction;

Research Use Only