ELISA Kit for Dystrophin Associated Glycoprotein 1 (DAG1) - USEC448Mu

ELISA Kit for Dystrophin Associated Glycoprotein 1 (DAG1)

Size: 96T

Catalogue Number: USEC448Mu-96

Citations, Manuals and MSDS Available upon request.

Target: Dystrophin Associated Glycoprotein 1 (DAG1)

Alternative Names: Dystroglycan 1; 156DAG; A3a; AGRNR; DAG; Alpha-Dystroglycan; Beta-Dystroglycan

Target Species: Mouse

Detection range: 0.156-10ng/mL

Sensitivity: 0.057ng/mL

Uniprot: Q62165

Gene ID: 13138

Featured Series Function: Detects protein (regular version)

Recommended/Predicted Sample Types: Tissue Homogenates, Cell Culture Supernates and other Biological Fluids

Assay Time: 3h

Method: Colormetric

Specificity: Reactive with Mouse DAG1 / Dystrophin Associated Glycoprotein 1

Detection principle: Double-antibody Sandwich

Sample Size: 100uL

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Dystrophin Associated Glycoprotein 1 (DAG1).

No significant cross-reactivity or interference between Dystrophin Associated Glycoprotein 1 (DAG1) and analogues was observed.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dystrophin Associated Glycoprotein 1 (DAG1) were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dystrophin Associated Glycoprotein 1 (DAG1) were tested on 3 different plates, 8 replicates in each plate.

CV(%) = SD/meanX100

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.

To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards;

2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;

3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;

4. Aspirate and wash 3 times;

5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;

6. Aspirate and wash 5 times;

7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;

8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dystrophin Associated Glycoprotein 1 (DAG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dystrophin Associated Glycoprotein 1 (DAG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dystrophin Associated Glycoprotein 1 (DAG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dystrophin Associated Glycoprotein 1 (DAG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Neuroscience; Bone metabolism;

Research Use Only