Product Description

ELISA Kit for Methionine (Met)

Sizes: 24T, 48T, 96T

Catalogue Numbers: CEG466Ge-24, CEG466Ge-48, CEG466Ge-96

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Featured Series Function: Detects small molecule

Method: Colormetric

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to methionine has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled methionine and unlabeled methionine (Standards or samples) with the pre-coated antibody specific to methionine. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of methionine in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of methionine in the sample.

Reactivity: General

Target: Methionine,

Assay Length: 2h

Sample Size: 50uL

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates, Other Biological Fluids

Detection Method: Competitive Inhibition

Detection Range: 246.9-20,000ng/mL

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Methionine (Met) were tested 20 times on one plate, respectively.Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Methionine (Met) were tested on 3 different plates, 8 replicates in each plate.CV(%) = SD/meanX100

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Assay Procedure: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Specificity: Reactive with General species Met / Methionine

Sensitivity: 84.3ng/mL

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Shelf Life: 12 months

Research Use Only