Product Description

Endoplasmin Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES2263-50, ES2263-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012],

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: HSP90B1, GRP94, TRA1, Endoplasmin, 94 kDa glucose-regulated protein, GRP-94, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1, gp96 homolog

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human GRP94. AA range:754-803

Isotype: IgG

Target: Endoplasmin

Specificity: Endoplasmin Polyclonal Antibody detects endogenous levels of Endoplasmin protein.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Blood, Brain, Liver, Lung, Platelet, Skin,

Observed Band (KD): 92kD

Fields: Protein processing in endoplasmic reticulum, PI3K-Akt signaling pathway, IL-17 signaling pathway, Estrogen signaling pathway, Thyroid hormone synthesis, Salmonella infection, Pathways in cancer, Chemical carcinogenesis - receptor activation, Prostate cancer, Lipid and atherosclerosis, Fluid shear stress and atherosclerosis

Function: Function: Molecular chaperone that functions in the processing and transport of secreted proteins. Has ATPase activity
PTM: Phosphorylated
Similarity: Belongs to the heat shock protein 90 family
Subcellular Location: Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Subunit: Homodimer, disulfide-linked. Component of an EIF2 complex at least composed of CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5 (By similarity). Part a large chaperone multiprotein complex comprising CABP1, DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX

Subcellular Location: Endoplasmic reticulum lumen Sarcoplasmic reticulum lumen Melanosome Identified by mass spectrometry in melanosome fractions from stage I to stage IV

Storage and Stability: -20°C/1 year

Gene Name: HSP90B1

Protein Name: Endoplasmin

Mouse Gene ID: 22027

Mouse SwissProt Number: P08113

Research Use Only