Product Description

eNOS (phospho Ser615) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES6400-50, ES6400-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: NOS3, Nitric oxide synthase, endothelial, Constitutive NOS, cNOS, EC-NOS, Endothelial NOS, eNOS, NOS type III, NOSIII

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human eNOS around the phosphorylation site of Ser615. AA range:581-630

Isotype: IgG

Target: NOS3

Specificity: Phospho-NOS3 (S615) Polyclonal Antibody detects endogenous levels of NOS3 protein only when phosphorylated at S615.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Platelets, placenta, liver and kidney,

Observed Band (KD): 130-140kD

Fields: Arginine biosynthesis, Arginine and proline metabolism, Metabolic pathways, Calcium signaling pathway, cGMP-PKG signaling pathway, HIF-1 signaling pathway, Sphingolipid signaling pathway, PI3K-Akt signaling pathway, VEGF signaling pathway, Apelin signaling pathway, Platelet activation, Estrogen signaling pathway, Oxytocin signaling pathway, Relaxin signaling pathway, Insulin resistance, AGE-RAGE signaling pathway in diabetic complications, Diabetic cardiomyopathy, Lipid and atherosclerosis, Fluid shear stress and atherosclerosis

Function: Catalytic Activity: L-arginine + n NADPH + n H(+) + m O(2) = citrulline + nitric oxide + n NADP(+)
Cofactor: Binds 1 FAD
Cofactor: Binds 1 FMN
Cofactor: Heme group
Cofactor: Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme
Enzyme Regulation: Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN
Function: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets
Online Information: Nitric oxide synthase entry,polymorphism: Variation in NOS3 seem to be associated with susceptibility to coronary spasm
Similarity: Belongs to the NOS family
Similarity: Contains 1 FAD-binding FR-type domain
Similarity: Contains 1 flavodoxin-like

Subcellular Location: Cell membrane Membrane, caveola Cytoplasm, cytoskeleton Golgi apparatus Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle, which is favored by interaction with NOSIP and results in a reduced enzymatic activity

Storage and Stability: -20°C/1 year

Gene Name: NOS3

Protein Name: Nitric oxide synthase endothelial

Mouse Gene ID: 18127

Mouse SwissProt Number: P70313

Rat Gene ID: 24600

Rat SwissProt Number: Q62600

Research Use Only