Product Description

EphA2 (phospho-Tyr772) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES16729-50, ES16729-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.[provided by RefSeq, May 2010],

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB

Alternate Names: Ephrin type-A receptor 2 (EC 2.7.10.1) (Epithelial cell kinase) (Tyrosine-protein kinase receptor ECK)

Clonality: Polyclonal

Immunogen: Synthesized phosho peptide around human EphA2 (Tyr772)

Isotype: IgG

Target: EphA2

Specificity: This antibody detects endogenous levels of Human EphA2 (phospho-Tyr772)

Dilution: WB 1:1000-2000

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in brain and glioma tissue and glioma cell lines (at protein level), Expressed most highly in tissues that contain a high proportion of epithelial cells, e,g, skin, intestine, lung, and ovary,

Observed Band (KD): 105kD

Fields: MAPK signaling pathway, Ras signaling pathway, Rap1 signaling pathway, PI3K-Akt signaling pathway, Axon guidance

Function: Catalytic Activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Function: Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 and -A5
Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily
Similarity: Contains 1 protein kinase domain
Similarity: Contains 1 SAM (sterile alpha motif) domain
Similarity: Contains 2 fibronectin type-III domains
Subunit: Interacts with SLA (By similarity). Interacts with INPPL1/SHIP2
Tissue Specificity: Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g
skin, intestine, lung, and ovary

Subcellular Location: Cell membrane, Single-pass type I membrane protein Cell projection, ruffle membrane, Single-pass type I membrane protein Cell projection, lamellipodium membrane, Single-pass type I membrane protein Cell junction, focal adhesion Present at regions of cell-cell contacts but also at the leading edge of migrating cells (PubMed:19573808, PubMed:20861311) Relocates from the plasma membrane to the cytoplasmic and perinuclear regions in cancer cells (PubMed:18794797)

Storage and Stability: -20°C/1 year

Gene Name: EPHA2 ECK

Protein Name: EphA2 (Tyr772)

Mouse Gene ID: 13836

Mouse SwissProt Number: Q03145

Research Use Only