F-Testo (Free Testosterone) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK9584-48, ELK9584-96, ELK9584-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: F-TESTO
Assay Type: Competitive Inhibition
Sensitivity: 18.76 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Areas: Endocrinology; Reproductive science; Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with F-TESTO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to F-TESTO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of F-TESTO in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only