Product Description

Firefly Luciferase Reporter Gene Luminescence Assay Kit (Flash Type)

Sizes: 48T, 96T

Catalogue Numbers: E-BC-F072-48, E-BC-F072-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Firefly Luciferin reporter gene detection is a reporting system that uses luciferin as a substrate to detect the activity of Firefly luciferase.
The detection principle of this kit: In the presence of oxygen, ATP and magnesium ions at the same time, firefly luciferin is catalyzed by firefly luciferin in the sample to oxidize luciferin and emit yellow-green light, and the expression of firefly luciferin in the sample can be detected by the chemiluminescence instrument.

Research Areas: Amino Acids and Proteins

Result Type: Quantitative

Detection Instrument: Chemiluminescence immunoassay analyzer or multifunctional microplate reader (with the function of detecting luminescence)

Detection Type: Fluorometric method

Tested Sample Types: Cell

Assay Length: 40 min

Inter cv: 5.3-6.8%

Intra cv: 2.3-4.5%

Research Use Only