Product Description

Fish Cor (Cortisol) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11732-48, ELK11732-96, ELK11732-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Fish

Alternate Names: Hydrocortisone

Sensitivity: 0.92 ng/mL

Assay Type: Competitive Inhibition

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: Serum, Plasma, Urine, Other Biological Fluids

Assay Length: 2h

Research Area: Metabolic Pathway, Endocrinology, Hormone Metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Fish Cor. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Fish Cor. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fish Cor in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only