Product Description

Fish Estrogen ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11828-48, ELK11828-96, ELK11828-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Fish

Alternate Names: E , Estrogen

Sensitivity: 4.15 pg/mL

Assay Type: Competitive Inhibition

Standard: 1000 pg/mL

Detection Range: 15.625-1000 pg/mL

Sample Type: Serum, Plasma, Tissue Homogenates, Other Biological Fluids

Assay Length: 2h

Research Area: Reproductive Science

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Fish Estrogen. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Fish Estrogen. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fish Estrogen in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only