Product Description

Flk-1/VEGFR2 (phospho Tyr1214) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1314-50, ES1314-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: KDR, FLK1, VEGFR2, Vascular endothelial growth factor receptor 2, VEGFR-2, Fetal liver kinase 1, FLK-1, Kinase insert domain receptor, KDR, Protein-tyrosine kinase receptor flk-1, CD antigen CD309

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human VEGFR2 around the phosphorylation site of Tyr1214. AA range:1180-1229

Isotype: IgG

Target: Flk-1/VEGFR2

Specificity: Phospho-Flk-1 (Y1214) Polyclonal Antibody detects endogenous levels of Flk-1 protein only when phosphorylated at Y1214.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Detected in cornea (at protein level), Widely expressed,

Observed Band (KD): 152kD

Fields: EGFR tyrosine kinase inhibitor resistance, MAPK signaling pathway, Ras signaling pathway, Rap1 signaling pathway, Calcium signaling pathway, PI3K-Akt signaling pathway, VEGF signaling pathway, Focal adhesion, Proteoglycans in cancer, Fluid shear stress and atherosclerosis

Function: Catalytic Activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Function: Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions
Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family
Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily
Similarity: Contains 1 protein kinase domain
Similarity: Contains 7 Ig-like C2-type (immunoglobulin-like) domains
Subunit: Interacts with MYOF (By similarity). Interacts with SHB, upon VEGF activation. Interacts with HIV-1 Tat

Subcellular Location: Cell junction Endoplasmic reticulum Cell membrane Localized with RAP1A at cell-cell junctions (By similarity) Colocalizes with ERN1 and XBP1 in the endoplasmic reticulum in endothelial cells in a vascular endothelial growth factor (VEGF)-dependent manner (PubMed:23529610), [Isoform 1]: Cell membrane, Single-pass type I membrane protein Cytoplasm Nucleus Cytoplasmic vesicle Early endosome Detected on caveolae-enriched lipid rafts at the cell surface Is recycled from the plasma membrane to endosomes and back again Phosphorylation triggered by VEGFA binding promotes internalization and subsequent degradation VEGFA binding triggers internalization and translocation to the nucleus, [Isoform 2]: Secreted, [Isoform 3]: Secreted

Storage and Stability: -20°C/1 year

Gene Name: KDR

Protein Name: Vascular endothelial growth factor receptor 2

Mouse SwissProt Number: P35918

Rat Gene ID: 25589

Rat SwissProt Number: O08775

Research Use Only