Product Description

Fluo-4 Calcium Fluorometric Assay Kit

Sizes: 96T

Catalogue Numbers: E-BC-F100-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: As an important metal ion in biological organism, calcium ion not only participates in the transmission of intracellular signals, but also plays an important role in many life activities of cells. Therefore, the detection of calcium ion concentration is of great significance in experimental research. This assay kit utilizes the cell-membrane-permeable calcium fluorescent probe Fluo-4 AM. The probe is non-fluorescent outside cells. Once inside cells, it is hydrolyzed by intracellular esterases to Fluo-4, which becomes trapped within the cytoplasm. Free Fluo-4 exhibits very weak fluorescence, but its fluorescence intensity increases significantly upon binding to Ca2+ . The probe shows maximum excitation at approximately 490 nm and maximum emission at 525 nm, and the fluorescence intensity is directly proportional to the intracellular calcium level.

Abbreviation: Ca

Research Areas: Inorganic Ions;Mitochondria Function

Result Type: Quantitative

Detection Instrument: Fluorescence microplate reader;Fluorescence microscope;Flow cytometry

Detection Type: Fluorometric method

Tested Sample Types: Cell

Assay Length: 1 h 30 min

Research Use Only