Product Description

Free Cholesterol (FC) Colorimetric Assay Kit

Sizes: 48T, 96T, 500Assays

Catalogue Numbers: E-BC-K004-M-48, E-BC-K004-M-96, E-BC-K004-M-500

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Free cholesterol produces 4-cholestenone and hydrogen peroxide under the oxidation of cholesterol oxidase. In the presence of 4-aminoamylpyridine and phenol, peroxidase catalyze hydrogen peroxide to form red quinone compounds of benzoquinone imine phenizone. The color depth of the generated quinones is directly proportional to the cholesterol content.

Abbreviation: FC

Target Synonym: FC

Research Areas: Glycolysis and Lipid Metabolism

Result Type: Quantitative

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Detection Range: 0.07-24 mmol/L

Tested Sample Types: Serum;Plasma;Tissue

Assay Length: 20 min

Sensitivity: 0.07 mmol/L

Inter cv: 5.2%

Intra cv: 1.9%

Research Use Only