Product Description

Free Fatty Acids (NEFA/FFA) Fluorometric Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-F039-48, E-BC-F039-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen peroxide in the presence of acyl oxidase. In the presence of the enzyme and probe, hydrogen peroxide react to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is directly proportional to the concentration of free fatty acids.

Abbreviation: NEFA

Target Synonym: FFA;NEFA

Research Areas: Metabolic Diseases;Glycolysis and Lipid Metabolism;Energy Metabolism

Result Type: Quantitative

Detection Instrument: Fluorescence microplate reader

Detection Type: Fluorometric method

Detection Range: 1.20-100 μmol/L

Tested Sample Types: Serum;Plasma;Animal tissue;Cell

Assay Length: 1 h 10 min

Sensitivity: 1.20 μmol/L

Inter cv: 5.1%

Intra cv: 4.1%

Research Use Only