Product Description

GABAA Rβ1 (phospho-S434) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS4078-50, BS4078-100

Swiss-Prot: P18505

Host: Rabbit

GABAA Rβ1 (phospho-S434) Polyclonal Antibody-BS4078

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: GAD-65 and GAD-67, glutamate decarboxylases, function to catalyze the production of GABA (gamma-aminobutyric acid). In the central nervous system GABA functions as the main inhibitory transmitter by increasing a Cl- conductance that inhibits neuronal firing. GABA has been shown to activate both ionotropic (GABAA) and metabotropic (GABAB) receptors as well as a third class of receptors called GABAC. Both GABAA and GABAC are ligand-gated ion channels, however, they are structurally and functionally distinct. Members of the GABAA receptor family include GABAA Rα1-6, GABAA R β1-3, GABAA Rγ1-3, GABAA Rδ, GABAA Rε, GABAA Rρ1 and GABAA Rρ2. The GABAB family is composed of GABAB R1α and GABAB R1β. GABA transporters have also been identified and include GABA T-1, GABA T-2 and GABA T-3 (also designated GAT-1, -2, and -3). The GABA transporters function to terminate GABA action.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-GABAA Rβ1 (S434) polyclonal antibody detects endogenous levels of GABAA Rβ1 protein when phosphorylated at Ser434.

Bioworld Molecular Weight: ~ 54 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of p-GABAA Rβ1 (S434) polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: Rat brain tissue lysate, Immunohistochemistry (IHC) analyzes of p-GABAA Rβ1 (S434) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Alternative Name: Gamma-aminobutyric acid receptor subunit beta-1; GABA(A) receptor subunit beta-1; GABRB1; GABAARβ1

Immunogen: Synthetic phosphopeptide derived from human GABAA Rβ1 around the phosphorylation site of Serine 434.

Modification: Phosphorylation

Conjugate: Unconjugated