Product Description

GADD34 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES8613-50, ES8613-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008],

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, ELISA

Alternate Names: Protein phosphatase 1 regulatory subunit 15A (Growth arrest and DNA damage-inducible protein GADD34) (Myeloid differentiation primary response protein MyD116 homolog)

Clonality: Polyclonal

Immunogen: Synthetic peptide from human protein at AA range: 430-490

Isotype: IgG

Target: GADD34

Specificity: The antibody detects endogenous GADD34

Dilution: WB 1:500-2000, ELISA 1:10000-20000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Brain,

Observed Band (KD): 73kD

Fields: Protein processing in endoplasmic reticulum

Function: Function: Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'
Induction: By methyl methanesulfonate and ionizing irradiation. By IL24 in melanoma cells, which induces apoptosis
Miscellaneous: The phosphatase activity of the PPP1R15A-PP1 complex toward EIF2S1 is specifically inhibited by Salubrinal, a drug that protects cells from endoplasmic reticulum stress
PTM: Phosphorylated on tyrosine by LYN, which impairs its antiproliferative activity
Similarity: Belongs to the PPP1R15 family
Subunit: Interacts with PCNA (By similarity)

Subcellular Location: Endoplasmic reticulum membrane, Peripheral membrane protein, Cytoplasmic side Mitochondrion outer membrane, Peripheral membrane protein, Cytoplasmic side Associates with membranes via an N-terminal amphipathic intramembrane region

Storage and Stability: -20°C/1 year

Gene Name: PPP1R15A GADD34

Protein Name: Protein phosphatase 1 regulatory subunit 15A (Growth arrest and DNA damage-inducible protein GADD34) (Myeloid differentiation primary response protein MyD116 homolog)

Mouse Gene ID: 17872

Mouse SwissProt Number: P17564

Research Use Only