Product Description

GalNAc4ST-1 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES7167-50, ES7167-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene belongs to the sulfotransferase 2 family. It is predominantly expressed in the pituitary gland, and is localized to the golgi membrane. This protein catalyzes the transfer of sulfate to position 4 of non-reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. It is responsible for sulfation of GalNAc on luteinizing hormone (LH), which is required for production of the sex hormones. Mice lacking this enzyme, exhibit increased levels of circulating LH, and precocious sexual maturation of both male and female mice. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2011],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: CHST8, Carbohydrate sulfotransferase 8, GalNAc-4-O-sulfotransferase 1, GalNAc-4-ST1, GalNAc4ST-1, N-acetylgalactosamine-4-O-sulfotransferase 1

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human CHST8. AA range:341-390

Isotype: IgG

Target: GalNAc4ST-1

Specificity: GalNAc4ST-1 Polyclonal Antibody detects endogenous levels of GalNAc4ST-1 protein.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Predominantly expressed in pituitary gland, In brain, it is expressed in pituitary gland, cerebellum, medulla oblongata, pons, thalamus and spinal cord, Expressed in the epidermis, Expressed at lower level in lung, spleen, adrenal gland, placenta, prostate, testis, mammary gland and trachea,

Observed Band (KD): 40kD

Fields: Various types of N-glycan biosynthesis, Metabolic pathways

Function: Caution: PubMed: 10988300 reports the possible existence of a secreted isoform starting at Met-119. However, they do not provide any experimental evidence
Function: Catalyzes the transfer of sulfate to position 4 of non-reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. Required for biosynthesis of glycoprotein hormones lutropin and thyrotropin, by mediating sulfation of their carbohydrate structures. Only active against terminal GalNAcbeta1,GalNAcbeta. Not active toward chondroitin
Induction: Down-regulated (17-fold) in prion-infected cells
Similarity: Belongs to the sulfotransferase 2 family
Tissue Specificity: Predominantly expressed in pituitary gland. In brain, it is expressed in pituitary gland, cerebellum, medulla oblongata, pons, thalamus and spinal cord. Expressed at lower level in lung, spleen, adrenal gland, placenta, prostate, testis, mammary glan

Subcellular Location: Golgi apparatus membrane, Single-pass type II membrane protein

Storage and Stability: -20°C/1 year

Gene Name: CHST8

Protein Name: Carbohydrate sulfotransferase 8

Mouse Gene ID: 68947

Mouse SwissProt Number: Q8BQ86

Research Use Only