GAPDH Rabbit mAb (High Dilution)
Size: 100μg
Catalogue Number: A19056-100
Citations, Manuals and MSDS Available upon request.
Background: This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD) . The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants..
Note：Due to the high antibody titer, it is advisable to be diluted before use. Please dilute 5μL of the antibody solution with 45μL of PBS solution, containing 50% glycerol. The diluted antibody can be stored at -20°C. Category: Loading Control Antibodies Applications: WB, IHC-P, IF/ICC, ELISA Cross-reactivity: Human, Mouse, Rat Protein Weight: 36kDa Observed Molecular Weight: 36kDa Immunogen: Recombinant protein (or fragment). Species: Human GeneID: 2597 SWISS: P04406 Alternate Names: G3PD; GAPD; HEL-S-162eP; GAPDH Source: Rabbit Isotype: IgG Purity: Affinity purification Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. Recommended Dilutions: WB, 1:50000 - 1:300000 IHC-P, 1:500 - 1:1000 IF/ICC, 1:50 - 1:200 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. Research Areas: Cancer, Signal Transduction, Endocrine Metabolism, Carbohydrate metabolism, Neuroscience, Neurodegenerative Diseases, Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimer's Disease. NCBI Alias: GAPDH Clone Number Info: ARC50888 Research Use Only
Note：Due to the high antibody titer, it is advisable to be diluted before use. Please dilute 5μL of the antibody solution with 45μL of PBS solution, containing 50% glycerol. The diluted antibody can be stored at -20°C. Category: Loading Control Antibodies Applications: WB, IHC-P, IF/ICC, ELISA Cross-reactivity: Human, Mouse, Rat Protein Weight: 36kDa Observed Molecular Weight: 36kDa Immunogen: Recombinant protein (or fragment). Species: Human GeneID: 2597 SWISS: P04406 Alternate Names: G3PD; GAPD; HEL-S-162eP; GAPDH Source: Rabbit Isotype: IgG Purity: Affinity purification Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. Recommended Dilutions: WB, 1:50000 - 1:300000 IHC-P, 1:500 - 1:1000 IF/ICC, 1:50 - 1:200 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. Research Areas: Cancer, Signal Transduction, Endocrine Metabolism, Carbohydrate metabolism, Neuroscience, Neurodegenerative Diseases, Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimer's Disease. NCBI Alias: GAPDH Clone Number Info: ARC50888 Research Use Only