Product Description

GCS-α-1 (D423) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3670-50, BS3670-100

Sizes: 50µl, 100µl

Swiss-Prot: Q02108

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Guanylate cyclases belong to the adenylyl cyclase class-4/guanylyl cyclase family. There are two forms of guanylate cyclase, a soluble form (GCS or sGC), which act as receptors for nitric oxide and a membrane-bound receptor form (GC), which are peptide hormone receptors. The GC-C protein is composed of an extracellular domain, a single transmembrane domain, and a cytoplasmic region consisting of a kinase-like domain and a catalytic domain. It is expressed as two differentially glycosylated forms, a 130 kDa precursor form present in the endoplasmic reticulum and a 145 kDa form present on the plasma membrane. Ligand binding to the extracellular domain of GC-C promotes the accumulation of cGMP. GC-C acts as the receptor for heatstable enterotoxins, small peptides secreted by some pathogenic strains of E. coli that cause severe secretory diarrhea. GC-C also binds to guanylin and uroguanylin peptides, which modulate renal function in response to oral salt load.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: GCS-α-1 (D423) polyclonal antibody detects endogenous levels of GCS-α-1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of GCS-α-1 (D423) pAb at 1:500 dilution
Lane 1: PC3 whole cell lysate (20ug)
Lane 2: A549 whole cell lysate (20ug)
Lane 3: HEK293T whole cell lysate (20ug)
Lane 4: PMVEC whole cell lysate (40ug)
Lane 5: The Brain tissue lysate of Mouse (40ug), Immunohistochemistry (IHC) analyzes of GCS-α-1 (D423) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 77 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Guanylate cyclase soluble subunit alpha-3; GCS-alpha-3; EC=4.6.1.2; GCS-alpha-1; Soluble guanylate cyclase large subunit; GUC1A3; GUCSA3; GUCY1A1; GUCY1A3; GCSα1; GCS-α1

Immunogen: Synthetic peptide, corresponding to amino acids 400-450 of Human GCS-α-1.

Conjugate: Unconjugated

Modification: Unmodified