Product Description

GFP-tag Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS6507-50, BS6507-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Green fluorescent protein (GFP) is a 27 a protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.

Category: Primary Antibody

Reactivity: Transfected

Host: Rabbit

Applications: WB, IF/ICC, IP

Alternate Names: Green fluorescent protein

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: Recombinant protein of fμll length GFP.

Dilution: WB 1:2000 - 1:5000
IF/ICC 1:200 - 1:500
IP 1:100 - 1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: GFP-tag polyclonal antibody detects over-expressed or recombinant proteins containing the GFP epitope tag.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only