Product Description

Glutathione Peroxidase (GSH-Px) Activity Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-K096-M-48, E-BC-K096-M-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Glutathione peroxidase (GSH-Px) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG). The activity of glutathione peroxidase can be expressed by the rate of enzymatic reaction. The activity of glutathione can be calculated by measuring the consumption of reduced glutathione. Hydrogen peroxide (H2O2) and reduced glutathione can react without catalysis of GSH-Px, so the portion of GSH reduction by non-enzymatic
reaction should be subtracted. GSH can react with dinitrobenzoic acid to produce a stable yellow substance. Measure the absorbance at 412 nm, and calculate the amount of GSH.

Abbreviation: GSH-Px

Target Synonym: GSH-PX

Research Areas: Oxidative stress;Plant Physiology Series;Ferroptosis

Result Type: Enzyme activity

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Detection Range: 34.34-1036.64 U/mL

Tested Sample Types: Serum;Plasma;Cell;Cell culture supernatant;Tissue

Assay Length: 40 min

Sensitivity: 34.34 U/mL

Inter cv: 8.7%

Intra cv: 2.4%

Research Use Only