Product Description

Glutathione-S-Transferase (GST) Activity Assay Kit (DTNB Method)

Sizes: 96T

Catalogue Numbers: E-BC-K800-M-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: GST can catalyze the binding of reduced glutathione (GSH) to dinitrobenzene (CDNB). The enzyme activity is indicated by measuring the substrate GSH binding rate with dinitrodiphenyl in unit time, the reaction of the rest of the GSH acts with disulfide double nitro benzoic acid (DTNB) to form yellow glucosinolates nitro benzoic acid anion (TNB), the concentration of which is determined to calculate the reduction of GSH. Thus, the activity of glutathione S-transferase (GST) was calculated indirectly by measuring the OD value at 412 nm.

Abbreviation: GST

Target Synonym: GST

Research Areas: Oxidative stress;Liver and Renal Function

Result Type: Enzyme activity

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Detection Range: 2.1-92.8 U/L

Tested Sample Types: Serum;Plasma;Animal tissue

Assay Length: 1 h 10 min

Sensitivity: 2.1 U/L

Inter cv: 6.4%

Intra cv: 1.8%

Research Use Only