Product Description

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Activity Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-K790-M-48, E-BC-K790-M-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), a key enzyme in the glycolytic pathway, catalyzes the oxidation of glyceraldehyde-3-phosphate to 1, 3-diphosphoglycerate. This response is closely related to the gluconeogenic pathway, the maintenance of blood glucose concentrations in the body, and the development of diabetes. GAPDH gene is a housekeeping gene, which is expressed at a high level in almost all tissues. Therefore, GAPDH widely exists in tissues and cells and plays an important role in the disorders of glucose, lipid and protein metabolism in the body.
This kit can calculate the enzyme activity of GAPDH by measuring the OD value change at 450 nm per unit time of the product produced by GAPDH catalyzing the oxidation of glyceraldehyde-3-phosphate.

Abbreviation: GAPDH

Target Synonym: GAPDH

Research Areas: Glycolysis and Lipid Metabolism;Energy Metabolism

Result Type: Enzyme activity

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Detection Range: 0.49-50.00 U/L

Tested Sample Types: Tissue;Cell

Assay Length: 50 min

Sensitivity: 0.49 U/L

Inter cv: 3.2-8.6%

Intra cv: 3.2-5.0%

Research Use Only