Product Description

Glycolysis Stress Fluorometric Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-F084-48, E-BC-F084-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Glycolysis is an important ATP generation pathway in eukaryotic cells. Glycolytic stress testing can directly measure the extracellular acidification rate (ECAR) to evaluate the changes of glycolytic process in living cells, and can obtain key parameters for a comprehensive assessment of glycolytic pathway, including: glycolysis, glycolytic capacity, glycolytic reserve and non-glycolytic acidification.
This kit uses 2-deoxy-D-glucose (2-DG) and oligomycin to facilitate in-depth analysis of cellular glycolytic flux: 2-DG quantifies nonglycolytic extracellular acidification (ECA) by blocking glycolysis through competitive hexokinase inhibition; Oligomycin inhibits ATP synthetase and therefore prevents aerobic ATP production, forcing the cell to increase glycolytic flux to meet ATP demand. Such compensatory glycolysis levels achievable under the test conditions could reveal glycolytic perturbations that are not apparent under basal conditions and help assess glycolytic capacity with limited ATP demand.

Research Areas: Energy Metabolism

Result Type: Quantitative

Detection Instrument: Fluorescence microplate reader

Detection Type: Fluorometric method

Tested Sample Types: Cell

Assay Length: 2 h

Research Use Only