Guinea pig AGTR1 (Angiotensin II Receptor 1) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK9549-48, ELK9549-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: AT1R; AG2S; AG2-S; AGTR1A; AGTR1B; AT1; AT1B; AT2R1; AT2-R1; AT2R1A; AT2R1B; HAT1R; AT1 Receptor; Angiotensin Receptor 1B
Species: Guinea pig
Assay Type: Sandwich
Sensitivity: 0.053 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue Homogenates and other Biological Fluids.
Assay length: 3.5h
Research Areas: Signal transduction; Metabolic pathway; Endocrinology; Cardiovascular biology; Hormone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guinea pig AGTR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig AGTR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guinea pig AGTR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guinea pig AGTR1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9WV26
Research Use Only.