Product Description

H+K+-ATPase Activity Assay Kit

Sizes: 100Assays, 200Assays

Catalogue Numbers: E-BC-K122-S-100, E-BC-K122-S-200

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: ATPase can decompose ATP to produce ADP and inorganic phosphorus. The activity of ATPase can be expressed by measuring the production amount of inorganic phosphorus in unit time. The inorganic phosphorus reacts with ammonium molybdate in acidic solution to form ammonium molybdate compound, which is reduced with reducing agent to form molybdenum blue, and has absorption peak at 660 nm. Determine the concentration of molybdenum blue to calculate the amount of inorganic phosphorus.

Research Areas: Oxidative Stress

Result Type: Enzyme activity

Detection Instrument: Spectrophotometer

Detection Type: Colorimetric method

Tested Sample Types: Animal tissue;Cell

Assay Length: 1 h

Inter cv: 9.8%

Intra cv: 4.4%

Research Use Only