Product Description

Hamster TNF-a (Tumor Necrosis Factor-alpha) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK0928-48, ELK0928-96, ELK0928-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hamster TNF-a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hamster TNF-a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hamster TNF-a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hamster TNF-a in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2

Reactivity: Hamster

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Cytokine, Tumor Immunity, Infection Immunity

Assay Length: 3.5h

Sensitivity: 1.09 pg/mL

Standard: 200 pg/mL

Detection Range: 3.13-200 pg/mL

Research Use Only