Product Description

HAND1 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES8040-50, ES8040-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: IF, ELISA

Alternate Names: HAND1, BHLHA27, EHAND, Heart- and neural crest derivatives-expressed protein 1, Class A basic helix-loop-helix protein 27, bHLHa27, Extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1, eH

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human HAND1. AA range:141-190

Isotype: IgG

Target: HAND1

Specificity: HAND1 Polyclonal Antibody detects endogenous levels of HAND1 protein.

Dilution: Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Heart,

Molecular Weight (Da): 24kD

Fields: Signaling pathways regulating pluripotency of stem cells

Function: Function: Plays an essential role in early trophoblast differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box)
Similarity: Contains 1 basic helix-loop-helix (bHLH) domain
Subunit: Efficient DNA binding requires dimerization with another bHLH protein. Forms homodimers and heterodimers with TCF3 gene products E12 and E47, HAND2 and HEY1, HEY2 and HEYL (hairy-related transcription factors)
Tissue Specificity: Heart

Subcellular Location: Nucleus, nucleoplasm Nucleus, nucleolus Interaction with MDFIC sequesters it into the nucleolus, preventing the transcription factor activity Phosphorylation by PLK4 disrupts the interaction with MDFIC and releases it from the nucleolus, leading to transcription factor activity (By similarity)

Storage and Stability: -20°C/1 year

Gene Name: HAND1

Protein Name: Heart- and neural crest derivatives-expressed protein 1

Mouse Gene ID: 15110

Mouse SwissProt Number: Q64279

Rat Gene ID: 59112

Rat SwissProt Number: P97832

Research Use Only