Product Description

High Sensitivity Human CFB (Complement Factor B) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11103HS-48, ELK11103HS-96, ELK11103HS-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Human

Alternate Names: CF-B, BF, BFD, CFAB, GBG, H2-Bf, PBF2, B-Factor, Properdin, Properdin factor B, C3/C5 convertase, Glycine-rich beta glycoprotein

Sensitivity: 10 pg/mL

Assay Type: Sandwich

Standard: 4000 pg/mL

Detection Range: 62.5-4000 pg/mL

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Length: 3.5h

Research Area: Infection immunity, Immune molecule, Hematology, Autoimmunity,

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CFB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CFB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CFB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CFB in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P00751

Research Use Only