Product Description

High Sensitivity Human I-PTH (IntactParathormone) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8878-48, ELK8878-96, ELK8878-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin; PTH; Parathyroid Hormone

Assay Type: Sandwich

Sensitivity: 0.9 pg/mL

Standard: 200 pg/mL

Detection Range: 3.13-200 pg/mL

Sample Type: serum, plasma and other biological fluids

Assay Length: 3.5h

Research Areas: Metabolic pathway; Endocrinology; Hormone metabolism;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human I-PTH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human I-PTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human I-PTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human I-PTH in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only