Product Description

High Sensitivity Mouse IL4 (Interleukin 4) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK1153HS-48, ELK1153HS-96, ELK1153HS-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Mouse

Alternate Names: IL-4, BSF1, BCGF1 , B Cell Stimulatory Factor 1, Lymphocyte stimulatory factor 1, B Cell Growth Factor, Binetrakin, Pitrakinra

Sensitivity: 2.2 pg/mL

Assay Type: Sandwich

Standard: 200 pg/mL

Detection Range: 3.13-200 pg/mL

Sample Type: Serum, Plasma, Cell Culture Supernates

Assay Length: 3.5h

Research Area: Cytokine, Immune molecule,

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL4 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P07750

Research Use Only