Product Description

High Sensitivity Rat MIP1a (Macrophage Inflammatory Protein 1 Alpha) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK1243HS-48, ELK1243HS-96, ELK1243HS-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Rat

Alternate Names: MIP1 Alpha, CCL3, MIP1-A, SCYA3, Chemokine C-C-Motif Ligand 3, Small Inducible Cytokine A3, Homologous To Mouse Mip-1a, Tonsillar Lymphocyte LD78 Alpha Protein

Sensitivity: 0.22 pg/mL

Assay Type: Sandwich

Standard: 250 pg/mL

Detection Range: 3.91-250 pg/mL

Sample Type: Serum, Plasma, Cell Culture Supernates

Assay Length: 3.5h

Research Area: Cytokine, Infection immunity,

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MIP1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MIP1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MIP1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MIP1a in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P50229

Research Use Only