Product Description

High Sensitivity Rat TIMP1 (Tissue Inhibitors Of Metalloproteinase 1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK10237HS-48, ELK10237HS-96, ELK10237HS-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Rat

Alternate Names: EPO, CLGI, EPA, HCI, Erythroid Potentiating Activity, Collagenase Inhibitor, Metalloproteinase inhibitor 1, Fibroblast collagenase inhibitor

Sensitivity: 0.073 pg/mL

Assay Type: Sandwich

Standard: 150 pg/mL

Detection Range: 2.34-150 pg/mL

Sample Type: Serum, Plasma

Assay Length: 3.5h

Research Area: Tumor Immunity, Infection Immunity, Cardiovascular Biology, Hepatology, Hormone Metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TIMP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TIMP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TIMP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TIMP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P30120

Research Use Only